University of Surrey 5 articles published in JoVE Bioengineering Ex Vivo Perfusion Culture of Large Blood Vessels in a 3D Printed Bioreactor Rolando S. Matos*1, Akram Jassim Jawad*1, Davide Maselli1, John H. McVey1, Christian Heiss1,2, Paola Campagnolo1 1Department of Biochemical Sciences, School of Biosciences, University of Surrey, 2Department of Clinical and Experimental Medicine, School of Biosciences, University of Surrey This protocol presents the setup and operation of a newly developed, 3D printed bioreactor for the ex vivo culture of blood vessels in perfusion. The system is designed to be easily adopted by other users, practical, affordable, and adaptable to different experimental applications, such as basic biology and pharmacological studies. Behavior Human Circadian Phenotyping and Diurnal Performance Testing in the Real World Elise R. Facer-Childs1,2,3, Benita Middleton1, Andrew P. Bagshaw2, Debra J. Skene1 1Chronobiology, Faculty of Health & Medical Sciences, University of Surrey, 2Centre for Human Brain Health, University of Birmingham, 3Turner Institute for Brain and Mental Health, School of Psychological Sciences, Monash University Here, we present a method to investigate diurnal rhythms in performance following accurate categorization of participants into circadian phenotype groups based on the Munich ChronoType Questionnaire, gold standard circadian phase biomarkers and actigraphic measures. Biochemistry An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes Valentina Iadevaia*1, Ana M. Matia-González*1, André P. Gerber1 1Dept. of Microbial Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey A tandem RNA isolation procedure (TRIP) for recovery of endogenously formed mRNA-protein complexes is described. Specifically, RNA-protein complexes are crosslinked in vivo, polyadenylated RNAs are isolated from extracts with oligo(dT) beads, and particular mRNAs are captured with modified RNA antisense oligonucleotides. Proteins bound to mRNAs are detected by immunoblot analysis. Engineering Flow-assisted Dielectrophoresis: A Low Cost Method for the Fabrication of High Performance Solution-processable Nanowire Devices Kaspar Snashall1, Marios Constantinou1, Maxim Shkunov1 1Advanced Technology Institute, Electrical and Electronic Engineering, University of Surrey In this paper, flow assisted dielectrophoresis is demonstrated for the self-assembly of nanowire devices. The fabrication of a silicon nanowire field effect transistor is shown as an example. Medicine Voluntary Breath-hold Technique for Reducing Heart Dose in Left Breast Radiotherapy Frederick R. Bartlett1, Ruth M. Colgan1, Ellen M. Donovan1, Karen Carr1, Steven Landeg1, Nicola Clements1, Helen A. McNair1, Imogen Locke1, Philip M. Evans2, Joanne S. Haviland3, John R. Yarnold4, Anna M. Kirby1 1Department of Radiotherapy, Royal Marsden NHS Foundation Trust, 2Centre for Vision, Speech and Signal Processing, Faculty of Engineering and Physical Sciences, University of Surrey, 3Clinical Trials and Statistics Unit (ICR-CTSU), Institute of Cancer Research, Sutton, UK, 4Division of Radiotherapy and Imaging, Institute of Cancer Research, Sutton, UK The current priority in breast cancer radiotherapy is to reduce cardiac doses without compromising target tissue coverage. The voluntary breath-hold technique described here is a simple, inexpensive solution to this problem and capable of being instituted widely without the need for specialized equipment.
