National Academy of Scientists Education program 2 articles published in JoVE Cancer Research Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery Isotta Sturniolo1,2, Csongor Váróczy1,3, Ákos Máté Bede1,3, Csaba Hegedűs1, Máté Á. Demény1,4, László Virág1,4 1Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, 2Doctoral School of Molecular Medicine, University of Debrecen, 3National Academy of Scientist Education, 4HUN-REN-DE Cell Biology and Signaling Research Group Here, we present a method to identify compounds that modulate the ADCC mechanism, an important cancer cell-killing mechanism of antitumor antibodies. The cytotoxic effect of NK cells is measured in breast cancer cell spheroids in the presence of Trastuzumab. Image analysis identifies live and dead killer and target cells in spheroids. Cancer Research High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds Eliza Guti*1,2, Ákos Máté Bede*1,3, Csongor Váróczy*1,3, Csaba Hegedűs1, Máté Á. Demény1,4, László Virág1,4 1Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, 2Doctoral School of Molecular Medicine, University of Debrecen, 3National Academy of Scientist Education, University of Debrecen, 4ELKH-DE Cell Biology and Signaling Research Group This protocol presents an automated, image-based high-throughput technique to identify compounds modulating natural killer cell-mediated breast cancer cell killing in the presence of a therapeutic anti-HER-2 antibody.
