Kaplan Medical Center 2 articles published in JoVE Cancer Research Implantation and Evaluation of Melanoma in the Murine Choroid via Optical Coherence Tomography Dimitri Gaber1, Michal Aharoni-Simon1, Ortal Zaks1, Keren Ben-Yaakov1, Ziv Rotfogel1,2, Hana Leiba1,2, Avital Eisenberg-Lerner*1, Arie L. Marcovich*1,2 1Ophthalmology Research Laboratory, Kaplan Medical Center, 2Department of Ophthalmology, Kaplan Medical Center, Faculty of Medicine, The Hebrew University of Jerusalem The present protocol describes the implantation and evaluation of melanoma in the murine choroid utilizing optical coherence tomography. Bioengineering Engineered Vascularized Muscle Flap Dana Egozi*1, Yulia Shandalov*2, Alina Freiman2,3, Dekel Rosenfeld2, David Ben-Shimol4, Shulamit Levenberg2 1Department of Plastic Surgery, Kaplan Medical Center, 2Biomedical Engineering, Technion Israel Institute of Technology, 3Interdepartmental Program in Biotechnology, Technion Israel Institute of Technology, 4Medicine Department, Technion Israel Institute of Technology To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps.