University of Turin View Institution's Website 8 articles published in JoVE Neuroscience Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis Brigitta Bonaldo1,2, Antonino Casile1,2,3, Francesca Montarolo1,2, Antonio Bertolotto1,4 1Neuroscience Institute Cavalieri Ottolenghi (NICO), 2Department of Neuroscience “Rita Levi-Montalcini”, University of Turin, 3School of Pharmacy, Pharmacology Unit, University of Camerino, 4Koelliker Hospital Experimental autoimmune encephalomyelitis is one of the most widely used murine models of multiple sclerosis. In the current protocol, C57BL/6J mice of both sexes are immunized with myelin oligodendrocyte glycoprotein peptide, resulting mainly in ascending paresis of the tail and limbs. Here we discuss the protocol of EAE induction and evaluation. Medicine Learning Modern Laryngeal Surgery in a Dissection Laboratory Erika Crosetti1, Marco Fantini1, Davide Lancini2, Andrea Manca1, Giovanni Succo1,3 1Head and Neck Oncology Unit, Candiolo Cancer Institute, FPO-IRCCS, 2Department of Otorhinolaryngology - Head and Neck Surgery, University of Brescia, 3Department of Oncology, University of Turin The purpose of this paper is to illustrate how to organize a reproducible laboratory for laryngeal surgery on affordable and closely similar animal laryngeal models in order to improve anatomical and surgical knowledge and skills. Environment Assessment of Methane and Nitrous Oxide Fluxes from Paddy Field by Means of Static Closed Chambers Maintaining Plants Within Headspace Chiara Bertora1, Matteo Peyron1, Simone Pelissetti2, Carlo Grignani1, Dario Sacco1 1Department of Agricultural, Forest and Food Sciences, University of Turin, 2UPTOFARM The overall goal of this protocol is to measure greenhouse gas emissions from paddy fields using the static closed chamber technique. The measurement system needs specific adjustments due to the presence of both a permanent water layer in the field and of the plants within the chamber headspace. Chemistry Synthesis and Characterization of Fe-doped Aluminosilicate Nanotubes with Enhanced Electron Conductive Properties Ehsan Shafia1, Serena Esposito2, Elnaz Bahadori1, Marco Armandi1,3, Maela Manzoli4, Barbara Bonelli1,3,5 1Department of Applied Science and Technology, Politecnico di Torino, 2Department of Civil and Mechanical Engineering, Università degli Studi di Cassino e del Lazio Meridionale, 3Institute of Chemistry, Politecnico di Torino, 4Department of Chemistry & NIS Interdepartmental Centre, University of Turin, 5INSTM Unit of Torino-Politecnico, Politecnico di Torino Here, we present a protocol to synthesize and characterize Fe-doped aluminosilicate nanotubes. The materials are obtained by either sol-gel synthesis upon addition of FeCl3•6H2O to the mixture containing the Si and Al precursors or by post-synthesis ionic exchange of preformed aluminosilicate nanotubes. Chemistry HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells Sara Serra1, Silvia Deaglio1 1Department of Medical Sciences, Human Genetics Foundation (HuGeF), University of Turin The protocol described here represents an easy and reproducible method that employs reverse phase high-performance liquid chromatography (RP-HPLC) to measure purine metabolism on chronic lymphocytic leukemia (CLL) cells cultured under different conditions. Developmental Biology Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression Cinzia M. Bertea1, Ravishankar Narayana1, Chiara Agliassa1, Christopher T. Rodgers2, Massimo E. Maffei1 1Department of Life Sciences and Systems Biology, University of Turin, 2Radcliffe Department of Medicine, University of Oxford, John Radcliffe Hospital This protocol describes a method to test the hypothesis that the reversal of the effective geomagnetic field (GMF) induces differential gene expression and alters the morphology of Arabidopsis thaliana. A triaxial octagonal system of Helmholtz coil-pairs was used to artificially generate reversed GMF conditions in the plant growth chamber. Neuroscience Non-invasive Parenchymal, Vascular and Metabolic High-frequency Ultrasound and Photoacoustic Rat Deep Brain Imaging Pierangela Giustetto1,2, Miriam Filippi2, Mauro Castano3, Enzo Terreno1,2 1Center for Preclinical Imaging, Department of Molecular Biotechnology and Health Sciences, University of Turin, 2Molecular Imaging Center, Department of Molecular Biotechnology and Health Sciences, University of Turin, 3Bracco Research Center, Bracco Imaging SpA The present work describes a new protocol to perform non-invasive high-frequency ultrasound and photoacoustic based imaging on rat brain, to efficiently visualize deep subcortical regions and their vascular patterns by directing signals on skull foramina naturally present on animal cranium. Medicine Photothrombotic Ischemia: A Minimally Invasive and Reproducible Photochemical Cortical Lesion Model for Mouse Stroke Studies Vivien Labat-gest1,2,3, Simone Tomasi2,3,4 1Department of Neuroscience, University of Turin, 2Neuroscience Institute of Turin (NIT), University of Turin, 3Neuroscience Institute Cavalieri-Ottolenghi (NICO), University of Turin, 4Department of Anatomy, Pharmacology and Forensic Medicine, University of Turin Photothrombosis is a quick, minimally-invasive technique for inducing small and well-delimited infarction in areas of interest in highly reproducible manner. It is particularly suitable for studying cellular and molecular responses underlying brain plasticity in transgenic mice.