IRCCS Istituto Ortopedico Galeazzi 3 articles published in JoVE Behavior Treatment of Ankle Osteoarthritis with Total Ankle Replacement Through a Lateral Transfibular Approach Federico G. Usuelli1, Riccardo D'Ambrosi1,2, Luigi Manzi1, Camilla Maccario1,2, Cristian Indino1 1Galeazzi Orthopedic Institute for Research and Care, 2Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano We present our preoperative, operative, and postoperative protocols for the treatment of osteoarthritis of the ankle with total ankle replacement via lateral transfibular approach. Medicine Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus Riccardo D'Ambrosi1,2, Cristian Indino1, Camilla Maccario1, Luigi Manzi1, Federico Giuseppe Usuelli1 1IRCCS Istituto Ortopedico Galeazzi - C.A.S.C.O. Piede e Caviglia, 2Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano The aim of this study is to report a protocol for the arthroscopic treatment of osteochondral lesions of the talus using microfractured and purified adipose-derived stem cells. Developmental Biology Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes Federica Franciosi1, Irene Tessaro1,2, Rozenn Dalbies-Tran3, Cecile Douet3, Fabrice Reigner4, Stefan Deleuze5, Pascal Papillier3, Ileana Miclea6, Valentina Lodde1, Alberto M. Luciano1, Ghylene Goudet3 1Department of Health, Animal Science and Food Safety, University of Milan, 2IRCCS. Istituto Ortopedico Galeazzi, 3PRC, CNRS, IFCE, Université de Tours, INRA, 4PAO, INRA, 5Clinique des Animaux de Compagnie et des Équidés, Université de Liège, 6University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania This manuscript describes an experimental approach to morphologically and biochemically characterize horse oocytes. Specifically, the present work illustrates how to collect immature and mature horse oocytes by ultrasound-guided ovum pick-up (OPU) and how to investigate chromosome segregation, spindle morphology, global histone acetylation, and mRNA expression.
