University of Naples Federico II 10 articles published in JoVE Engineering A Single-Channel and Non-Invasive Wearable Brain-Computer Interface for Industry and Healthcare Pasquale Arpaia1,2, Antonio Esposito1,3, Nicola Moccaldi1,4, Marco Parvis5 1Department of Electrical Engineering and Information Technology (DIETI), University of Naples Federico II, 2Interdepartmental Center for Research and Innovation in Management and Healthcare (CIRMIS), University of Naples Federico II, 3Advanced Metrological Services Center (CeSMA), University of Naples Federico II, 4Department of Innovation Engineering, University of Salento, 5Department of Electronics and Telecommunication (DET), Polytechnic of Turin This paper discusses how to build a brain-computer interface by relying on consumer-grade equipment and steady-state visually evoked potentials. For this, a single-channel electroencephalograph exploiting dry electrodes was integrated with augmented reality glasses for stimuli presentation and output data visualization. The final system was non-invasive, wearable, and portable. Environment Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution Giovanni Gallo1,2, Martina Aulitto1,3, Patrizia Contursi1,4, Danila Limauro1,4, Simonetta Bartolucci1, Gabriella Fiorentino1,4 1Department of Biology, University of Naples Federico II, 2Institute of Polymers, Composites and Biomaterials (IPCB), Consiglio Nazionale delle Ricerche CNR, 3Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, 4BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Napoli Federico II The isolation of heavy metal-resistant microbes from geothermal springs is a hot topic for the development of bioremediation and environmental monitoring biosystems. This study provides a methodological approach for isolating and identifying heavy metal tolerant bacteria from hot springs. Chemistry Early Detection of Cyanobacterial Blooms and Associated Cyanotoxins using Fast Detection Strategy Roberta Teta1, Germana Esposito1, Carlo De Sterlich2, Massimiliano Lega*3, Valeria Costantino*1 1TheBlueChemistryLab, Department of Pharmacy, University of Naples Federico II, 2Department of Public Health, University of Naples Federico II, 3Department of Engineering, University Parthenope A fast-multidisciplinarystrategy for early detection of cyanobacterial blooms and associated cyanotoxins is described here. It allows for the detection of cyanobacteria and related cyanotoxins in water samples and in organic matrices, such as bivalve samples, in 24 h. Immunology and Infection A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice Sonia Simonetti*1,2, Ambra Natalini*1,2, Giovanna Peruzzi3, Alfredo Nicosia4, Antonella Folgori5, Stefania Capone5, Angela Santoni2,6, Francesca Di Rosa1 1Institute of Molecular Biology and Pathology, National Research Council of Italy (CNR), 2Department of Molecular Medicine, University of Rome “Sapienza”, 3Center for Life Nano Science, Istituto Italiano di Tecnologia, 4Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 5Reithera Srl, 6IRCCS, Neuromed Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated mice. Bioengineering Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method Immacolata Belviso*1, Anna Maria Sacco*1, Veronica Romano1, Fabrizio Schonauer1, Daria Nurzynska1, Stefania Montagnani1, Franca Di Meglio1, Clotilde Castaldo1 1Department of Public Health, University of Naples Federico II Cell reprogramming requires the introduction of key genes, which regulate and maintain the pluripotent cell state. The protocol described enables the formation of induced pluripotent stem cells (iPSCs) colonies from human dermal fibroblasts without viral/integrating methods but using non-modified RNAs (NM-RNAs) combined with immune evasion factors reducing cellular defense mechanisms. Developmental Biology Methods to Test Endocrine Disruption in Drosophila melanogaster Tiziana Francesca Bovier1, Daniela Cavaliere1, Michele Colombo1, Gianfranco Peluso2, Ennio Giordano3, Filomena Anna Digilio1,2 1Institute of Biosciences and BioResources, National Research Council of Italy (CNR), 2Institute of Research on Terrestrial Ecosystems (IRET), National Research Council of Italy (CNR), 3Department of Biology, UniNa "Federico II" Endocrine disruptor chemicals (EDCs) represent a serious problem for organisms and for natural environments. Drosophila melanogaster represents an ideal model to study EDC effects in vivo. Here, we present methods to investigate endocrine disruption in Drosophila, addressing EDC effects on fecundity, fertility, developmental timing, and lifespan of the fly. Biochemistry Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods Roberta Imperatore1,2, Livia D'Angelo3, Paolo De Girolamo3, Luigia Cristino2, Marina Paolucci1 1Department of Sciences and Technologies (DST), University of Sannio, 2Endocannabinoid Research Group, Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions, University of Naples Federico II Presented here are protocols for immunohistochemical characterization and localization of orexin peptide, orexin receptors, and endocannabinoid receptors in the gut and brains of normal and diet-induced obesity (DIO) adult zebrafish models using immunoperixidase and double immunofluorescence methods. Developmental Biology HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis Maria Teresa Gentile1, Olga Pastorino1, Maurizio Bifulco2, Luca Colucci-D'Amato1 1Laboratory of Cellular and Molecular Neuropathology, Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania "Luigi Vanvitelli", 2Department of Molecular Medicine and Biotechnologies, University of Naples "Federico II" Naples Here, we evaluate the effects of the water extract of Ruta graveolens on vessel network formation by using a tube formation assay on a gelled basement matrix. Immunology and Infection Spore Adsorption as a Nonrecombinant Display System for Enzymes and Antigens Rachele Isticato1, Ezio Ricca1, Loredana Baccigalupi2 1Department of Biology, Federico II University, 2Department of Molecular Medicine and Medical Biotechnology, University of Naples "Federico II" This protocol focuses on the use of bacterial spores as a "live" nanobiotechnological tool to adsorb heterologous molecules with various biological activities. The methods to measure the efficiency of adsorption are also shown. Behavior Assessing the Influence of Personality on Sensitivity to Magnetic Fields in Zebrafish Alessandro Cresci1, Rosario De Rosa2,3, Claudio Agnisola4 1Rosenstiel School of Marine and Atmospheric Science, Department of Ocean Sciences, University of Miami, 2Department of Physics, Monte S. Angelo (MSA) Campus, University of Naples Federico II, 3Istituto Nazionale di Fisica Nucleare (INFN), 4Department of Biology, MSA Campus, University of Naples Federico II We describe a behavioral protocol designed to assess how zebrafish’s personalities influence their response to water currents and weak magnetic fields. Fishes with the same personalities are separated based on their explorative behavior. Then, their rheotactic orientation behavior in a swimming tunnel with a low flow rate and under different magnetic conditions is observed.
