CEINGE, Biotecnologie Avanzate s.c.ar.l. 1 article published in JoVE Immunology and Infection Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery Chiara Pagliuca1, Elena Scaglione1, Francesca Carraturo1, Giuseppe Mantova1, Maria Michela Marino1, Maria Virginia Pishbin1, Caterina Pagliarulo2, Roberta Colicchio1, Paola Salvatore1,3 1Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 2Department of Science and Technology, Sannio University, 3CEINGE – Advanced Biotechnology Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues.
