IRCCS Ospedale Galeazzi Sant'Ambrogio 1 article published in JoVE Medicine Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy Martino Guiotto*1,4, Silvia Palombella*2, Stefania Brambilla2, Lee Ann Applegate3, Mathis Riehle4, Andrew Hart4, Wassim Raffoul1, Pietro Giovanni di Summa1 1Department of Plastic, Reconstructive and Hand Surgery, Centre Hospitalier Universitaire Vaudois (CHUV, 2 Xenogeneic (chemical or animal-derived) products introduced in the cell therapy preparation/manipulation steps are associated with an increased risk of immune reactivity and pathogenic transmission in host patients. Here, a complete xenogeneic-free method for the isolation and in vitro expansion of human adipose-derived stem cells is described.
