Neuroscience Institute Cavalieri Ottolenghi (NICO) 1 article published in JoVE Neuroscience Modeling Multiple Sclerosis in the Two Sexes: MOG35-55-Induced Experimental Autoimmune Encephalomyelitis Brigitta Bonaldo1,2, Antonino Casile1,2,3, Francesca Montarolo1,2, Antonio Bertolotto1,4 1Neuroscience Institute Cavalieri Ottolenghi (NICO), 2Department of Neuroscience “Rita Levi-Montalcini”, University of Turin, 3School of Pharmacy, Pharmacology Unit, University of Camerino, 4Koelliker Hospital Experimental autoimmune encephalomyelitis is one of the most widely used murine models of multiple sclerosis. In the current protocol, C57BL/6J mice of both sexes are immunized with myelin oligodendrocyte glycoprotein peptide, resulting mainly in ascending paresis of the tail and limbs. Here we discuss the protocol of EAE induction and evaluation.
