University Medical Center Groningen View Institution's Website 10 articles published in JoVE Medicine Determining and Controlling External Power Output During Regular Handrim Wheelchair Propulsion Rick de Klerk1, Riemer J. K. Vegter1, Marika T. Leving1, Sonja de Groot1,2, DirkJan H. E. J. Veeger3,4, Lucas H. V. van der Woude1,5 1Center for Human Movement Sciences, University Medical Center Groningen, 2Amsterdam Rehabilitation Research Center Reade, Amsterdam, 3Research Institute MOVE, Faculty of Behavioral and Human Movement Sciences, VU University, 43M, Delft University of Technology, Delft, 5Center for Rehabilitation, University Medical Center Groningen Accurate and standardized assessment of external power output is crucial in the evaluation of physiological, biomechanical, and perceived stress, strain, and capacity in manual wheelchair propulsion. The current article presents various methods to determine and control power output during wheelchair propulsion studies in the laboratory and beyond. Medicine Observational Study Protocol for Repeated Clinical Examination and Critical Care Ultrasonography Within the Simple Intensive Care Studies Renske Wiersema1, Jose N. Castela Forte1, Thomas Kaufmann2, Robbert J. de Haas3, Geert Koster1, Yoran M. Hummel4, Jacqueline Koeze1, Casper F. M. Franssen5, Madelon E. Vos1, Bart Hiemstra1, Frederik Keus1, Iwan C.C. van der Horst1 1Department of Critical Care, University of Groningen, University Medical Center Groningen, 2Department of Anesthesiology, University of Groningen, University Medical Center Groningen, 3Department of Radiology, University of Groningen, University Medical Center Groningen, 4Department of Cardiology, University of Groningen, University Medical Center Groningen, 5Division of Nephrology, Department of Internal Medicine, University of Groningen, University Medical Center Groningen Structured protocols are necessary to provide answers on research questions in critically ill patients. The Simple Intensive Care Studies (SICS) provides an infrastructure for repeated measurements in critically ill patients including clinical examination, biochemical analysis and ultrasonography. SICS projects have specific focus but the structure is flexible to other investigations. Immunology and Infection Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform Michele Perni1, Sam Casford1, Francesco A. Aprile1, Ellen A. Nollen2, Tuomas P.J. Knowles1, Michele Vendruscolo1, Christopher M. Dobson1 1Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, 2European Research Institute for the Biology of Aging, University Medical Centre Groningen We describe protocols for using the wide field-of-view nematode tracking platform (WF-NTP), which enables high-throughput phenotypic characterization of large populations of Caenorhabditis elegans. These protocols can be used to characterize subtle behavioral changes in mutant strains or in response to pharmacological treatment in a highly scalable fashion. Behavior An Automated Method to Determine the Performance of Drosophila in Response to Temperature Changes in Space and Time Andrea Soto-Padilla1,2, Rick Ruijsink3, Mark Span4, Hedderik van Rijn*4, Jean-Christophe Billeter*1 1Groningen Institute for Evolutionary Life Sciences, University of Groningen, 2Department of Cell Biology, University of Groningen, University Medical Center Groningen, 3Ruijsink Dynamic Engineering, 4Department of Psychology, University of Groningen Here we present a protocol to automatically determine the locomotor performance of Drosophila at changing temperatures using a programmable temperature-controlled arena that produces fast and accurate temperature changes in time and space. Developmental Biology Induction and Validation of Cellular Senescence in Primary Human Cells Alejandra Hernandez-Segura1, Simone Brandenburg1, Marco Demaria1 1European Research Institute for the Biology of Aging, University of Groningen, University Medical Center Groningen Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models. Medicine Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension Diederik E. van der Feen1, Michel Weij2, Annemieke Smit-van Oosten2, Lysanne M. Jorna1, Quint A.J. Hagdorn1, Beatrijs Bartelds1, Rolf M.F. Berger1 1Center for Congenital Heart Diseases, Department of Pediatric Cardiology, Beatrix Children's Hospital, University Medical Center Groningen, University of Groningen, 2Research and Development Facility, University Medical Center Groningen, University of Groningen This protocol describes a surgical procedure to create a model for flow-induced pulmonary arterial hypertension (PAH) in rats and the procedures to analyze the principle hemodynamic and histological end-points in this model. Medicine Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery Rick Pleijhuis1, Arwin Timmermans1, Johannes De Jong1, Esther De Boer1, Vasilis Ntziachristos2, Gooitzen Van Dam1 1Department of Surgery, University Medical Center Groningen, 2Helmholtz Zentrum Munich, Technical University of Munich Near-infrared fluorescence (NIRF) imaging may improve therapeutic outcome of breast cancer surgery by enabling intraoperative tumor localization and evaluation of surgical margin status. Using tissue-simulating breast phantoms containing fluorescent tumor-simulating inclusions, potential clinical applications of NIRF imaging in breast cancer patients can be assessed for standardization and training purposes. Bioengineering Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast Daphne H. E. W. Huberts1, Georges E. Janssens2, Sung Sik Lee3, Ima Avalos Vizcarra4, Matthias Heinemann1,5 1Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 2Department for the Biology of Ageing, European Research Institute for the Biology of Ageing, University Medical Centre Groningen, University of Groningen, 3Institute of Biochemistry, ETH Zurich, 4Department of Health Sciences and Technology, ETH Zurich, 5Institute of Molecular Systems Biology, ETH Zurich We describe here the operation of a microfluidic device that allows continuous and high-resolution microscopic imaging of single budding yeast cells during their complete replicative and/or chronological lifespan. Medicine The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage Annelien N. Morks1, Klaas Havenga1, Henk O. ten Cate Hoedemaker1, Rutger J. Ploeg1 1Department of Surgery, Division of Abdominal Surgery, University Medical Center Groningen The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown. Medicine Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology Lucia M.A. Crane1, George Themelis2, K. Tim Buddingh1, Niels J. Harlaar1, Rick G. Pleijhuis1, Athanasios Sarantopoulos2, Ate G.J. van der Zee3, Vasilis Ntziachristos2, Gooitzen M. van Dam1 1Department of Surgery, Division of Surgical Oncology, University Medical Center Groningen, 2Helmholtz Zentrum, Technical University Munich, 3Department of Obstetrics and Gynaecology, University Medical Center Groningen Fluorescence imaging is a promising innovative modality for image-guided surgery in surgical oncology. In this video we describe the technical procedure for detection of the sentinel lymph node using fluorescence imaging as showcased in gynecologic oncologicy. A multispectral fluorescence camera system, together with the fluorescent agent indocyanine green, is applied.
