Nencki Institute of Experimental Biology 3 articles published in JoVE Chemistry Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis Lukasz Bozycki1, Magdalena Komiazyk1, Saida Mebarek2,3,4,5,6, Rene Buchet2,3,4,5,6, Slawomir Pikula1, Agnieszka Strzelecka-Kiliszek1 1Laboratory of Lipid Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 2Université de Lyon, 3Université Lyon 1, 4 We present a protocol to compare the state of minerals in vesicles released by two human bone cell lines: hFOB 1.19 and Saos-2. Their mineralization profiles were analyzed by Alizarin Red-S (AR-S) staining, ultraviolet (UV) light visualization, transmission electron microscopy (TEM) imaging and energy dispersive X-ray microanalysis (EDX). Neuroscience Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex Kacper Łukasiewicz*1, Magdalena Robacha*1, Łukasz Bożycki2, Kasia Radwanska1, Rafał Czajkowski3 1Department of Molecular and Cellular Neuroscience, Nencki Institute of Experimental Biology, 2Department of Biochemistry, Nencki Institute of Experimental Biology, 3Neurobiology Centre, Nencki Institute of Experimental Biology This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC. Biology A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis Anna K. Schellhaus*1, Adriana Magalska*2, Allana Schooley1, Wolfram Antonin1 1Friedrich Miescher Laboratory, Max Planck Society, 2Nencki Institute of Experimental Biology, Polish Academy of Sciences The molecular mechanisms of the decondensation of highly compacted mitotic chromatin are ill-defined. We present a cell-free assay based on mitotic chromatin clusters isolated from HeLa cells and Xenopus laevis egg extract that faithfully reconstitutes the decondensation process in vitro.
