Maria Curie-Sklodowska University 4 articles published in JoVE Biochemistry RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing Natalia Kopik1,2, Olga Chrobak1,2, Przemyslaw Latoch1,3, Mariia Kovalenko1,2, Agata L. Starosta1,2 1Laboratory of Gene Expression, ECOTECH-Complex, Maria Curie-Sklodowska University, 2Department of Molecular Biology, Institute of Biological Sciences, Maria Curie-Sklodowska University, 3Faculty of Information Technology, Polish-Japanese Academy of Information Technology Here we describe the stages of sample collection and preparation for RIBO-seq in bacteria. Sequencing of the libraries prepared according to these guidelines results in sufficient data for comprehensive bioinformatic analysis. The protocol we present is simple, uses standard laboratory equipment and takes seven days from lysis to obtaining libraries. Medicine Magnetic Resonance Imaging of Multiple Sclerosis at 7.0 Tesla Sonia Waiczies*1, Antje Els*1, Joseph Kuchling*2,3,4, Karin Markenroth Bloch5, Anna Pankowska6,7, Helmar Waiczies8, Carl Herrmann1, Claudia Chien2,3, Carsten Finke4,9, Friedemann Paul2,3,4, Thoralf Niendorf1,2,8 1Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 2Experimental and Clinical Research Center, Max Delbrueck Center for Molecular Medicine and Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3NeuroCure Clinical Research Center, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 4Department of Neurology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 5The Swedish National 7T Facility, Lund University Bioimaging Center, Lund University, 6Department of Radiography, Medical University of Lublin, 7ECOTECH-COMPLEX, Maria Curie-Skłodowska University, 8MRI.TOOLS GmbH, 9Berlin School of Mind and Brain, Humboldt-Universität zu Berlin Here, we present a protocol to acquire magnetic resonance (MR) images of multiple sclerosis (MS) patient brains at 7.0 Tesla. The protocol includes preparation of the setup including the radio-frequency coils, standardized interview procedures with MS patients, subject positioning in the MR scanner and MR data acquisition. Behavior Measuring Light-Switching Behavior Using an Occupancy and Light Data Logger Krzysztof J. Leoniak*1, Wojciech Cwalina*1 1Department of Social Psychology, Maria Curie-Skłodowska University This article describes a procedure for using and deploying an occupancy and light data logger which allows collecting data about light-switching behavior of participants in field settings. Chemistry Surface Properties of Synthesized Nanoporous Carbon and Silica Matrices Angelina Sterczyńska1, Małgorzata Śliwińska-Bartkowiak1,2, Małgorzata Zienkiewicz-Strzałka3, Anna Deryło-Marczewska3 1Faculty of Physics, Adam Mickiewicz University, 2NanoBioMedical Centre, 3Department of Physicochemistry of Solid Surface, Faculty of Chemistry, Maria Curie-Skłodowska University Here we report the synthesis and characterization of ordered nanoporous carbon (with a 4.6 nm pore size) and SBA-15 (with a 5.3 nm pore size). The work describes the surface and textural properties of nanoporous molecular sieves, their wettability, and the melting behavior of D2O confined in the materials.
