Maria Sklodowska-Curie National Research Institute of Oncology 1 article published in JoVE Biology Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers Maciej Wakula1, Anna Balcerak1, Urszula Smietanka1, Mateusz Chmielarczyk1, Ryszard Konopiński1, Ewa A. Grzybowska1 1Maria Sklodowska-Curie National Research Institute of Oncology The article describes quantification of 1) the size and number of focal adhesions and 2) cell shape index and its distribution from confocal images of the confluent monolayers of MCF7 cells.