Universidade Catolica Portuguesa 1 article published in JoVE Medicine Micro-Colony Forming Unit Assay for Efficacy Evaluation of Vaccines Against Tuberculosis David Pires1,2,3, Paulo J. G. Bettencourt1,2 1Faculty of Medicine, Universidade Católica Portuguesa, 2Center for Interdisciplinary Research in Health, Universidade Católica Portuguesa, 3Host-Pathogen Interactions Unit, Research Institute for Medicines, iMed-ULisboa, Faculty of Pharmacy, Universidade de Lisboa The determination of colony-forming units (CFU) is the gold-standard technique for quantifying bacteria, including Mycobacterium tuberculosis which can take weeks to form visible colonies. Here we describe a micro-CFU for CFU determination with increased time efficiency, reduced lab space and reagent cost, and scalability to medium and high throughput experiments.
