National Laboratory of Health, Environment and Food 1 article published in JoVE Environment Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples José Gonçalves1,2,3, Priscilla Gomes da Silva4,5,6, Tom Koritnik3, Martin Bosilj3, Andrés Torres-Franco1,2, Israel Diaz1,2, Elisa Rodriguéz1,2, Eva Marcos2,3, João R. Mesquita4,5, Pedro García-Encina1,2 1Institute of Sustainable Processes, University of Valladolid, 2Department of Chemical Engineering and Environmental Technology, University of Valladolid, 3Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, 4ICBAS - School of Medicine and Biomedical Sciences, Porto University, 5Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR) This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization.
