National Institute of Biology 1 article published in JoVE Biology Studying Cell Death Initiation Using a Digital Microscope Tina Arnšek*1, Nuša Golob*1, Nastja Marondini1, Maruša Pompe-Novak1,2, Kristina Gruden1, Tjaša Lukan1 1National Institute of Biology, 2University of Nova Gorica Here we present a protocol for studying the rate of programmed cell death initiation by continuously imaging inoculated leaves following cell death induction.