Ludwig Boltzmann Institute for Cancer Research (LBI-CR) 1 article published in JoVE Cancer Research Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression Herwig P. Moll1, Julian Mohrherr2, Kristina Breitenecker2, Marcel Haber2, Viktor Voronin2, Emilio Casanova1,2 1Department of Physiology, Center of Physiology and Pharmacology & Comprehensive Cancer Center (CCC), Medical University of Vienna, 2Ludwig Boltzmann Institute for Cancer Research (LBI-CR) Here we describe a minimally invasive syngeneic orthotopic transplantation model of mouse lung adenocarcinoma cells as a time- and cost-reducing model to study non-small cell lung cancer.