Doctoral School of the University of Vienna and Medical University of Vienna 1 article published in JoVE Developmental Biology Protocol for Human Blastoids Modeling Blastocyst Development and Implantation Harunobu Kagawa*1, Alok Javali*1, Heidar Heidari Khoei*1, Theresa Maria Sommer1,3, Giovanni Sestini1,3, Maria Novatchkova2, Yvonne Scholte op Reimer1, Nicolas Rivron1 1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), 2Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.