3 articles published in JoVE
Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique Benno Cardini*1, Rupert Oberhuber*1, Sven R Hein1, Rebecca Eiter1, Martin Hermann2, Markus Kofler1,3, Stefan Schneeberger1, Gerald Brandacher1,4, Manuel Maglione1 1Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck, 2Department of Anesthesiology and Critical Care Medicine, Medical University Innsbruck, 4Department of Cardiac Surgery, Medical University Innsbruck, 3Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA) Laboratory, Johns Hopkins University School of Medicine Among abdominal solid organ transplantation, pancreatic grafts are prone to develop severe ischemia reperfusion injury-associated graft damage, leading eventually to early graft loss. This protocol describes a model of murine pancreas transplantation using a non-suture cuff technique, ideally suited for analyzing these early, deleterious damages.
Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies Roman Crazzolara1, Gabriele Kropshofer1, Michael Steurer2, Sieghart Sopper2,3, Wolfgang Schwinger4 1Department of Pediatrics, Medical University Innsbruck, 2Department of Internal Medicine V (Hematology & Oncology), Medical University Innsbruck, 3Tyrolean Cancer Research Institute, 4Department of Pediatrics, Medical University Graz Quantification of donor-derived cells is required to monitor engraftment after stem cell transplantation in patients with hemoglobinopathies. A combination of flow cytometry-based cell sorting, colony formation assay, and subsequent analysis of short tandem repeats may be used to assess the proliferation and differentiation of progenitors in the erythroid compartment.
Orthotopic Hind-Limb Transplantation in Rats Robert Sucher*1, Rupert Oberhuber*1, Christian Margreiter1, Guido Rumberg1, Rishi Jindal2, WP Andrew Lee2, Raimund Margreiter1, Johann Pratschke1, Stefan Schneeberger1, Gerald Brandacher1 1Department of Visceral, Transplant, and Thoracic Surgery, Daniel Swarovski Research Laboratory, Innsbruck Medical University, 2Department of Surgery, Division of Plastic and Reconstructive Surgery, University of Pittsburgh Medical Center Here we describe the orthotopic rat hind-limb transplantation procedure, which seems to be the gold standard in vivo model for composite tissue allotransplantation research.