3 articles published in JoVE
Cellular Redox Profiling Using High-content Microscopy Tom Sieprath1,2, Tobias Corne1,2, Joke Robijns1, Werner J. H. Koopman3, Winnok H. De Vos1,2 1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center This paper presents a high-content microscopy workflow for simultaneous quantification of intracellular ROS levels, as well as mitochondrial membrane potential and morphology – jointly referred to as mitochondrial morphofunction – in living adherent cells using the cell-permeant fluorescent reporter molecules 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA) and tetramethylrhodamine methylester (TMRM).
Functional Magnetic Resonance Imaging (fMRI) with Auditory Stimulation in Songbirds Lisbeth Van Ruijssevelt1, Geert De Groof1, Anne Van der Kant1, Colline Poirier1, Johan Van Audekerke1, Marleen Verhoye1, Annemie Van der Linden1 1Bio-Imaging Lab, University of Antwerp This article shows an optimized procedure for imaging of the neural substrates of auditory stimulation in the songbird brain using functional Magnetic Resonance Imaging (fMRI). It describes the preparation of the sound stimuli, the positioning of the subject and the acquisition and subsequent analysis of the fMRI data.
Multimodal Imaging of Stem Cell Implantation in the Central Nervous System of Mice Nathalie De Vocht1,2, Kristien Reekmans1, Irene Bergwerf2, Jelle Praet1,2, Chloé Hoornaert1, Debbie Le Blon1, Jasmijn Daans1, Zwi Berneman1, Annemie Van der Linden2, Peter Ponsaerts1 1Laboratory of Experimental Hematology, University of Antwerp, 2Bio Imaging Lab, University of Antwerp This article describes an optimized sequence of events for multimodal imaging of cellular grafts in rodent brain using: (i) in vivo bioluminescence and magnetic resonance imaging, and (ii) post mortem histological analysis. Combining these imaging modalities on a single animal allows cellular graft evaluation with high resolution, sensitivity and specificity.