Ghent University Hospital 6 articles published in JoVE Medicine Retzius-Sparing Robot-Assisted Radical Prostatectomy Nicolaas Lumen*1, Edward Lambert*1, Filip Poelaert1, Michel Wirtz1, Sofie Verbeke2, Charles Van Praet1 1Dept. of Urology, Ghent University Hospital, 2Dept. of Pathology, Ghent University Hospital Robot-assisted Retzius-sparing radical prostatectomy is a technique that enables to preserve urinary continence or facilitates recovery of urinary continence in the majority of patients. Patients must be informed about the risk of a positive surgical margin. Medicine Vessel-sparing Excision and Primary Anastomosis Wesley Verla1, Willem Oosterlinck1, Marjan Waterloos1, Nicolaas Lumen1 1Department of Urology, Ghent University Hospital Here, we present an elaborate and efficient protocol to treat isolated short bulbar or posterior urethral strictures with vessel-sparing excision and primary anastomosis. Cancer Research PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator Julie Bolcaen1, Benedicte Descamps2, Tom Boterberg3, Christian Vanhove2, Ingeborg Goethals1 1Department of Nuclear Medicine, Ghent University Hospital, 2IBiTech-MEDISIP, Department of Electronics and Information Systems, Ghent University, 3Department of Radiation Oncology, Ghent University Hospital In the past, small animal irradiation was usually performed without the ability to target a well-delineated tumor volume. The goal was to mimic the treatment of human glioblastoma in rats. Using a small animal irradiation platform, we performed MRI-guided 3D conformal irradiation with PET-based sub-volume boosting in a preclinical setting. Developmental Biology Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange Tim Pieters1,2,3,4, Lieven Haenebalcke1,2, Kenneth Bruneel1,2,4, Niels Vandamme1,2,4, Tino Hochepied1,2, Jolanda van Hengel5, Dagmar Wirth6, Geert Berx1,2,4, Jody J. Haigh7, Frans van Roy1,2,4, Steven Goossens1,2,3,4 1Department of Biomedical Molecular Biology, Ghent University, 2Inflammation Research Center, VIB, 3Center for Medical Genetics, Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology, Monash University and Alfred Health Alfred Centre Proteins often contain multiple domains that can exert different cellular functions. Gene knock-outs (KO) do not consider this functional diversity. Here, we report a recombination-mediated cassette exchange (RMCE)-based structure-function approach in KO embryonic stem cells that allows for the molecular dissection of various functional domains or variants of a protein. Immunology and Infection An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen Srinath Govindarajan1,2, Dirk Elewaut1,2, Michael Drennan1,2 1Department of Rheumatology, Laboratory for Molecular Immunology and Inflammation, Ghent University Hospital, 2VIB Inflammation Research Center, Ghent University Here we present an adapted protocol that can be used to generate a large number of murine invariant natural killer T cells from mouse spleen. The protocol outlines an approach by which splenic iNKT cells can be enriched for, isolated and expanded in vitro using a limited number of animals and reagents. Medicine The In ovo CAM-assay as a Xenograft Model for Sarcoma Gwen M.L. Sys1, Lore Lapeire2, Nikita Stevens1, Herman Favoreel3, Ramses Forsyth4, Marc Bracke2, Olivier De Wever2 1Department of Orthopaedic Surgery and Traumatology, Ghent University Hospital, 2Department of Radiation Oncology and Experimental Cancer Research, Ghent University, 3Department of Virology, Parasitology, and Immunology, Ghent University, 4Pathlicon The in ovo chorioallantoic membrane (CAM) is grafted with fresh sarcoma-derived tumor tissues, their single cell suspensions, and permanent and transient fluorescently labeled established sarcoma cell lines. The model is used to study graft- (viability, Ki67 proliferation index, necrosis, infiltration) and host (fibroblast infiltration, vascular ingrowth) behavior.
