2 articles published in JoVE
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time Katrijn R. Six1,2, Rosalie Devloo1, Britt Van Aelst1, Philippe Vandekerckhove2,3,4, Hendrik B. Feys1, Veerle Compernolle1,2,3 1Transfusion Research Center, Belgian Red Cross-Flanders, 2Faculty of Medicine and Health Sciences, Ghent University, 3Blood Service, Belgian Red Cross-Flanders, 4Department of Public Health and Primary Care, KULeuven - University of Leuven This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.
Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro Britt Van Aelst1, Hendrik B. Feys1, Rosalie Devloo1, Philippe Vandekerckhove2,3,4, Veerle Compernolle1,2,4 1Transfusion Research Center, Belgium Red Cross-Flanders, 2Blood Service, Belgium Red Cross-Flanders, 3Department of Public Health and Primary Care, Catholic University of Leuven, 4Faculty of Medicine and Health Sciences, University of Ghent Platelet transfusion and hemostasis was modeled using blood reconstitution and microfluidic flow chambers to investigate the function of blood banking platelets. The data demonstrate the consequences of platelet storage lesion on hemostasis, in vitro.