VIB-KU Leuven Center for Brain and Disease Research 1 article published in JoVE Neuroscience Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors Shelly Fripont*1,2, Catherine Marneffe*1,2, Marika Marino*1,2, Melvin Y. Rincon1,2, Matthew G. Holt1,2,3 1VIB-KU Leuven Center for Brain and Disease Research, 2Department of Neuroscience, KU Leuven, 3Leuven Brain Institute Here, we describe an efficient and reproducible strategy to produce, titer, and quality-control batches of adeno-associated virus vectors. It allows the user to obtain a vector preparation with high-titer (≥1 x 1013 vector genomes/mL) and a high purity, ready for in vitro or in vivo use.
