Swiss Institute for Experimental Cancer Research 1 article published in JoVE Biology In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors Chiara Greggio*1, Filippo De Franceschi*1, Manuel Figueiredo-Larsen*2, Anne Grapin-Botton1,2 1Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Swiss Institute for Experimental Cancer Research, 2DanStem, University of Copenhagen The three-dimensional culture method described in this protocol recapitulates pancreas development from dispersed embryonic mouse pancreas progenitors, including their substantial expansion, differentiation and morphogenesis into a branched organ. This method is amenable to imaging, functional interference and manipulation of the niche.