3 articles published in JoVE
BtM, a Low-cost Open-source Datalogger to Estimate the Water Content of Nonvascular Cryptogams María Leo*1, Angel Lareo*2, Carlos Garcia-Saura2, Joaquín Hortal3,4, Nagore G. Medina5 1Real Jardín Botánico (CSIC-RJB), 2Grupo de Neurocomputación Biológica, Dpto. de Ingeniería Informática, Escuela Politécnica Superior, Universidad Autónoma de Madrid, 3Department of Biogeography and Global Change, Museo Nacional de Ciencias Naturales (MNCN-CSIC), 4Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências da Universidade de Lisboa, 5Department of Botany, Faculty of Science, University of South Bohemia We present a simple and cost-effective method to build an open-source datalogger that measures the conductance of nonvascular cryptogams together with the environmental temperature and humidity. We describe the hardware design of the datalogger and provide step-by-step assembly instructions, the list of required open-source logging software, the code to run the datalogger, and a calibration protocol.
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei Ondřej Gahura1, Alena Zíková1,2 1Biology Centre, Czech Academy of Science, Institute of Parasitology, 2Faculty of Science, University of South Bohemia This protocol describes the purification of F1-ATPase from the cultured insect stage of Trypanosoma brucei. The procedure yields a highly pure, homogeneous, and active complex suitable for structural and enzymatic studies.
Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches Jia-Hsin Huang1, Yun Liu1, Yu-Hsien Lin1,2,3, Xavier Belles4, How-Jing Lee1 1Department of Entomology, National Taiwan University, 2Biology Centre, Institute of Entomology, Czech Academy of Sciences, 3Faculty of Science, University of South Bohemia, 4Institute of Evolutionary Biology, CSIC-UPF This manuscript demonstrates the depletion of gene expression in the midgut of the German cockroach through oral ingestion of double-stranded RNA encapsulated in liposomes.