T. G. Masaryk Water Research Institute 1 article published in JoVE Environment Bioindication Testing of Stream Environment Suitability for Young Freshwater Pearl Mussels Using In Situ Exposure Methods Michal Bílý1, Simona Němčíková1, Ondřej P. Simon1,2, Karel Douda3, Vojtěch Barák1, Bohumil Dort1 1Faculty of Environmental Sciences, Czech University of Life Sciences Prague, 2T. G. Masaryk Water Research Institute, 3Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague In situ bioindications enable determination of the suitability of an environment for endangered mussel species. We describe two methods based on the juvenile exposure of freshwater pearl mussels in cages to oligotrophic river habitats. Both methods are implemented in variants for open water and hyporheic water environments.