3 articles published in JoVE
iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution Julian Konig1, Kathi Zarnack2, Gregor Rot3, Tomaz Curk3, Melis Kayikci1, Blaz Zupan3, Daniel J. Turner4, Nicholas M. Luscombe2, Jernej Ule1 1Laboratory of Molecular Biology, Medical Research Council - MRC, 2European Bioinformatics Institute, EMBL Heidelberg, 3Computer and Information Science, University of Ljubljana, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute The spatial arrangement of RNA-binding proteins on a transcript is a key determinant of post-transcriptional regulation. Therefore, we developed individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) that allows precise genome-wide mapping of the binding sites of an RNA-binding protein.
Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy Benjamin Dale1, Gregory P. McNerney2, Deanna L. Thompson2, Wolfgang Hübner3, Thomas Huser2, Benjamin K. Chen1 1Division of Infectious Diseases, Department of Medicine, Immunology Institute, Mount Sinai School of Medicine, 2NSF Center for Biophotonics, University of California, Davis, 3Structural and Computational Biology Unit, European Molecular Biology Laboratory This visualized experiment is a guide for utilizing a fluorescent molecular clone of HIV for live confocal imaging experiments.
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid Ann-Marie Lawrence1, Hüseyin Besir1 1Protein Expression and Purification Core Facility, EMBL Heidelberg A short protocol for protein staining with Coomassie Brilliant Blue (CBB) G-250 in polyacrylamide gels is described without using organic solvents or acetic acid as in the classical staining procedures with CBB.