3 articles published in JoVE

• Biochemistry

  
  A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample Mohamed Salem^1,2, Michal Bernach¹, Krzysztof Bajdzienko¹, Patrick Giavalisco^{1 1}Max Planck Institute of Molecular Plant Physiology, Golm, Germany, ²Department of Pharmacognosy, Faculty of Pharmacy, Cairo University A protocol for comprehensive extraction of lipids, metabolites and proteins from biological tissues using one sample is presented.

• Biochemistry

  
  High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE Toshio Moriya¹, Michael Saur¹, Markus Stabrin¹, Felipe Merino¹, Horatiu Voicu², Zhong Huang², Pawel A. Penczek², Stefan Raunser¹, Christos Gatsogiannis^{1 1}Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, ²Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston This paper presents a protocol for processing cryo-EM images using the software suite SPHIRE. The present protocol can be applied for nearly all single particle EM projects that target near-atomic resolution.

• Biology

  
  A Protocol for the Identification of Protein-protein Interactions Based on ¹⁵N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation Stefan Schmollinger^1,2, Daniela Strenkert^1,2, Vittoria Offeddu^1,2, André Nordhues^1,2, Frederik Sommer^1,2, Michael Schroda^{1,2 1}Max Planck Institute of Molecular Plant Physiology, ²University of Kaiserslautern We present a variation of the QUICK (QUantitative Immunoprecipitation Combined with Knockdown) approach that was introduced previously to distinguish between true and false protein-protein interactions. Our approach is based on ^15N metabolic labeling, the modulation of affinities of protein-protein interactions by the presence/absence of ATP, immunoprecipitation, and quantitative mass spectrometry.