3 articles published in JoVE
Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells Alfonso Rodríguez-Gil1, Tabea Riedlinger2, Olesja Ritter2, Vera V. Saul2, M. Lienhard Schmitz2 1Department of Oncohematology and Genetics. Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, 2Institute of Biochemistry, Medical Faculty, Friedrichstrasse 24, Member of the German Center for Lung Research, Justus-Liebig-University The plasticity of nuclear architecture is controlled by dynamic epigenetic mechanisms including non-coding RNAs, DNA methylation, nucleosome repositioning as well as histone composition and modification. Here we describe a Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) protocol which allows the determination of chromatin accessibility in a reproducible, inexpensive, and straightforward manner.
Isolation and Cultivation of Adult Rat Cardiomyocytes Franziska Nippert1, Rolf Schreckenberg1, Klaus-Dieter Schlüter1 1Institute of Physiology, Justus Liebig University Giessen Here, we present a protocol for the isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). Isolated ARVC can be used for short and long-term cultivation. The isolation and cultivation of ARVC can play a key role in developing new treatment regimens for cardiac diseases.
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines Benedetto Daniele Giaimo1, Francesca Ferrante1, Tilman Borggrefe1 1Institute of Biochemistry, University of Giessen This work describes a protocol for chromatin immunoprecipitation (ChIP) using a mature mouse T-cell line. This protocol is suitable to investigate the distribution of specific histone marks at specific promoter sites or genome-wide.