2 articles published in JoVE
Isolation and Cultivation of Adult Rat Cardiomyocytes Franziska Nippert1, Rolf Schreckenberg1, Klaus-Dieter Schlüter1 1Institute of Physiology, Justus Liebig University Giessen Here, we present a protocol for the isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). Isolated ARVC can be used for short and long-term cultivation. The isolation and cultivation of ARVC can play a key role in developing new treatment regimens for cardiac diseases.
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines Benedetto Daniele Giaimo1, Francesca Ferrante1, Tilman Borggrefe1 1Institute of Biochemistry, University of Giessen This work describes a protocol for chromatin immunoprecipitation (ChIP) using a mature mouse T-cell line. This protocol is suitable to investigate the distribution of specific histone marks at specific promoter sites or genome-wide.