2 articles published in JoVE
Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK Rodrigo Lerchundi*1, Karl W. Kafitz*1, Marcel Färfers1, Felix Beyer2, Na Huang1, Christine R. Rose1 1Institute of Neurobiology, Heinrich Heine University Düsseldorf, 2Department of Neurology, Düsseldorf University Hospital We describe a protocol for cell-type specific expression of the genetically encoded FRET-based sensor ATeam1.03YEMK in organotypic slice cultures of the mouse forebrain. Furthermore, we show how to use this sensor for dynamic imaging of cellular ATP levels in neurons and astrocytes.
Using the Sleeve Technique in a Mouse Model of Aortic Transplantation - An Instructional Video Zuzanna Rowinska1,2, Simone Gorressen3, Marc W. Merx4, Thomas A. Koeppel5, Alma Zernecke6, Elisa A. Liehn2 1Department of Vascular Surgery and Interdisciplinary Vein Center, St. Josef-Hospital, Ruhr- University Bochum, 2Institute of Molecular Cardiovascular Research, University Hospital, RWTH Aachen University Department of Medicine, 3Institute of Pharmacology and Clinical Pharmacology, Düsseldorf University Hospital, 4Department of Cardiology, Vascular Medicine and Intensive Care Medicine, Hospital Robert Koch Gehrden, 5Division of Vascular Surgery, Asklepios Klinik St. Georg, 6Institute of Experimental Biomedicine, Würzburg University We present an orthotopic aortic transplantation model using the sleeve technique in mice. It is a very rapid anastomosis method, which can be employed in studies of vascular disease.