Joint Heidelberg-IDC Translational Diabetes Program 1 article published in JoVE Neuroscience Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry Asa S. Hidmark1, Peter P. Nawroth1,2,3, Thomas Fleming1,3 1Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, 2Institute for Diabetes and Cancer IDC Helmholtz Center Munich, Germany & Joint Heidelberg-IDC Translational Diabetes Program, 3German Center for Diabetes Research (DZD) Quantitative analysis of cell content within the murine sciatic nerve is difficult due to the scarcity of the tissue. This protocol describes a method for tissue digestion and preparation that provides sufficient cells for flow cytometry analysis of immune cell populations from nerves of individual mice.
