3 articles published in JoVE
Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue Nikaïa Smith1,2,3,4, Nassima Bekaddour2,3,4, Nicolas Leboulanger4,5, Yolande Richard*6, Jean-Philippe Herbeuval*2,3,4 1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin In the present protocol, we explain how to easily process and culture tonsillar mononuclear cells from healthy humans undergoing partial surgical tonsillectomy to study innate immune responses upon activation, mimicking viral infection in mucosal tissues.
Improved Registration of 3D CT Angiography with X-ray Fluoroscopy for Image Fusion During Transcatheter Aortic Valve Implantation Ina Vernikouskaya1, Wolfgang Rottbauer1, Julia Seeger1, Birgid Gonska1, Jochen Wöhrle*1, Volker Rasche*1 1Department of Internal Medicine II - Cardiology, Ulm University Medical Center The aim of this study was to improve the co-registration for image fusion (IF) of pre-interventional CT data with real-time x-ray (XR) fluoroscopy during transfemoral transcatheter aortic valve implantation (TAVI).
In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur Melanie Haffner-Luntzer1, Fabian Müller-Graf1,4, Romano Matthys2, Alireza Abaei3, René Jonas1, Florian Gebhard4, Volker Rasche*3, Anita Ignatius*1 1Institute of Orthopedic Research and Biomechanics, University Medical Center Ulm, 2RISystem, 3Core Facility Small Animal MRI, University Medical Center Ulm, 4Department of Traumatology, Hand-, Plastic-, and Reconstructive Surgery, University Medical Center Ulm The evaluation of tissue development in the fracture callus during endochondral bone healing is essential to monitor the healing process. Here, we report the use of a magnetic resonance imaging (MRI)-compatible external fixator for the mouse femur to allow MRI scans during bone regeneration in mice.