3 articles published in JoVE
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells Olivia Krefft*1, Ammar Jabali*1, Vira Iefremova1, Philipp Koch1,2,3, Julia Ladewig1 1Institute of Reconstructive Neurobiology, University of Bonn, 2Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH) Cerebral organoids represent a new model system to investigate early human brain development in vitro. This article provides the detailed methodology to efficiently generate homogeneous dorsal forebrain-type organoids from human induced pluripotent stem cells including critical characterization and validation steps.
Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature Lucas Moritz Wiggenhauser1, Katharina Kohl2, Nadine Dietrich2, Hans-Peter Hammes2, Jens Kroll1 1Department of Vascular Biology and Tumorangiogenesis, Center for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim, Heidelberg University, 2V. Medical Clinic, Medical Faculty Mannheim, Heidelberg University Here, we discuss a method protocol which will allow an easy analysis of the adult tg(fli:EGFP) zebrafish retinal vasculature as a fast read-out in settings of long-term vascular pathologies linked to neoangiogenesis and structural changes.
Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks Henning D. Popp1, Susanne Brendel1, Wolf-Karsten Hofmann1, Alice Fabarius1 1Department of Hematology and Oncology, Medical Faculty Mannheim of the Heidelberg University This manuscript provides a protocol for the analysis of DNA double-strand breaks by immunofluorescence microscopy of γH2AX and 53BP1.