3 articles published in JoVE
Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting Frauke Hausburg*1,2, Paula Müller*1,2, Natalia Voronina*1, Gustav Steinhoff1,2, Robert David1,2 1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery, Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty, Rostock University This protocol illustrates a safe and efficient procedure to modify CD133+ hematopoietic stem cells. The presented non-viral, magnetic polyplex-based approach may provide a basis for the optimization of therapeutic stem cell effects as well as for monitoring the administered cell product via magnetic resonance imaging.
In Vitro Enzyme Measurement to Test Pharmacological Chaperone Responsiveness in Fabry and Pompe Disease Jan Lukas1, Anne-Marie Knospe1, Susanne Seemann1, Valentina Citro2, Maria V. Cubellis2, Arndt Rolfs1,3 1Albrecht-Kossel-Institute, University Rostock Medical Center, 2Department of Biology, University Federico II, 3Centogene AG There is a demand to make pre-clinical testing for a novel class of "orphan" drugs called pharmacological chaperones reproducible, fast, and efficient. We developed a simple, highly standardized, and versatile cell culture-based assay to screen for eligible patients as well as novel pharmacological chaperone drugs.
Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse Daniel Strüder*1, Eberhard Grambow*2, Ernst Klar2, Robert Mlynski1, Brigitte Vollmar3 1Department of Otorhinolaryngology, Head and Neck Surgery "Otto Koerner", Rostock University Medical Center, 2Department of General, Thoracic, Vascular and Transplantation Surgery, Rostock University Medical Center, 3Institute for Experimental Surgery, Rostock University Medical Center The ear model of the hairless SKH1-Hrhr mouse enables intravital fluorescence microscopy of microcirculation and phototoxic thrombus induction without prior surgical preparation in the examined microvascular bed. Therefore, the ear of the hairless mouse is an excellent in vivo model to study the complex interactions during microvascular thrombus formation, thrombus evolution, and thrombolysis.