3 articles published in JoVE
A Volumetric Method for Quantification of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage Axel Neulen*1, Michael Kosterhon*1, Tobias Pantel*1, Stefanie Kirschner2, Hermann Goetz3, Marc A. Brockmann2, Sven R. Kantelhardt1, Serge C. Thal4 1Department of Neurosurgery, Medical Center of the Johannes Gutenberg - University, 2Department of Neuroradiology, Medical Center of the Johannes Gutenberg - University, 3Platform for Biomaterial Research, Medical Center of the Johannes Gutenberg - University, 4Department of Anesthesiology, Medical Center of the Johannes Gutenberg - University The goal of this article is to present a method that allows a 3-dimensional reconstruction of the cerebrovascular tree in mice after micro computed tomography and determination of volumes of entire vessel segments that can be used to quantify cerebral vasospasm in murine models of subarachnoid hemorrhage.
Topical Airway Anesthesia for Awake-endoscopic Intubation Using the Spray-as-you-go Technique with High Oxygen Flow Nina Pirlich1, Jana A. Lohse1, Rüdiger R. Noppens1 1Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University The topical anesthetic lidocaine was atomized using a high oxygen flow through the working channel of a flexible intubating endoscope to achieve topical airway anesthesia for awake endotracheal intubation. We prefer this modified spray-as-you-go technique for endoscopic intubation to classical bolus application because of higher patient satisfaction and better compliance.
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues Imen Jebeniani1, Julia Leschik2, Michel Puceat1 1INSERM UMR-910, Faculté de Médecine, Université Aix-Marseille, 2Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg University A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.