3 articles published in JoVE
Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities Johannes Lambrecht1, Florian Schattenberg1, Hauke Harms1, Susann Mueller1 1Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ Flow cytometric analysis has proven valuable for investigating pure cultures and monitoring microbial community dynamics. We present three comprehensive workflows, from sampling to data analysis, for pure cultures and complex communities in clear medium as well as in challenging matrices, respectively.
A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems Susan Schamfuß1, Thomas R. Neu2, Hauke Harms1, Lukas Y. Wick1 1Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, 2Department of River Ecology, Helmholtz Centre for Environmental Research - UFZ A whole cell bioreporter assay with Burkholderia sartisoli RP037-mChe was developed to detect fractions of an organic contaminant (i.e., fluorene) available for bacterial degradation after active transport by mycelia bridging air-filled pores in a water unsaturated model system.
Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization Carla Gimkiewicz1, Falk Harnisch1 1Department of Environmental Microbiology, UFZ - Helmholtz-Centre for Environmental Research Chronoamperometric growth of anodic electroactive microbial biofilms in a fed-batch reactor using a three-electrode setup, controlled by a potentiostat, is demonstrated. The extracellular electron transfer is characterized using cyclic voltammetry in the presence of the electron donor and in the absence of the electron donor. Fundamental data analysis is demonstrated.