2 articles published in JoVE
Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers Joseph D. Unsay1,2,3, Katia Cosentino1,2, Ana J. García-Sáez1,2 1Interfaculty Institute for Biochemistry, 2Max Planck Institute for Intelligent Systems, 3German Cancer Research Center We describe a protocol for preparation of supported lipid bilayers and its characterization using atomic force microscopy and force spectroscopy.
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach Theresa L. M. Pohl1, Elisabeth H. Schwab1, Elisabetta A. Cavalcanti-Adam1,2 1Department of Biophysical Chemistry, Institute for Physical Chemistry, University of Heidelberg, 2Max Planck Institute for Intelligent Systems at Stuttgart We describe a method for accomplishing efficient immobilization of BMP-2 on surfaces. Our approach is based on the formation of a self-assembled monolayer to achieve the covalent binding of BMP-2 via its free amine residues. This method is a useful tool to study signaling at the cell membrane.