Jena University Hospital

9 articles published in JoVE

• Immunology and Infection

  
  Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry Susann Hartung^1,2, Christopher Rauh², Sarah Böttcher¹, Mai Thi Ngoc Hoang^1,2, Susanne Jahreis^1,2, Silke Rummler³, Andreas Hochhaus², Marie von Lilienfeld-Toal^{1,2 1}Infections in Hematology and Oncology, Leibniz Institute for Infection Biology and Natural Product Research, ²Department for Hematology and Medical Oncology, Jena University Hospital, ³Institute for Transfusion Medicine, Jena University Hospital This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes.

• Bioengineering

  
  A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat An Wang¹, Isabel Jank¹, Weiwei Wei¹, Claudia Schindler¹, Uta Dahmen^{1 1}Experimental Transplantation Surgery, Department of General, Visceral and Vascular Surgery, University Hospital Jena We establish a novel surgical technique for an in vivo single liver lobe perfusion model in rat as a prerequisite for further studying in vivo partial liver engineering in the future.

• Medicine

  
  Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice Chichi Xie¹, Weiwei Wei¹, Andrea Schenk³, Lars Ole Schwen³, Sara Zafarnia², Michael Schwier³, Felix Gremse², Isabel Jank¹, Olaf Dirsch⁴, Uta Dahmen^{1 1}Department of General, Visceral and Vascular Surgery, Jena University Hospital, ²Experimental Molecular Imaging, RWTH Aachen University, ³Fraunhofer Institute for Medical Image Computing MEVIS, ⁴Institute of Pathology, Klinikum Chemnitz gGmbH Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using µCT and histological serial sections.

• Chemistry

  
  Investigations on the Ga(III) Complex of EOB-DTPA and Its ⁶⁸Ga Radiolabeled Analogue Julia Greiser¹, Tobias Niksch¹, Wolfgang Weigand², Martin Freesmeyer^{1 1}Clinic of Nuclear Medicine, University Hospital Jena, ²Friedrich Schiller University A procedure for the isolation of EOB-DTPA and subsequent complexation with natural Ga(III) and ⁶⁸Ga is presented herein, as well as a thorough analysis of all compounds and investigations on labeling efficiency, in vitro stability and the n-octanol/water distribution coefficient of the radiolabeled complex.

• Neuroscience

  
  Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis Anne Ansorg*¹, Katja Bornkessel*¹, Otto W. Witte¹, Anja Urbach^{1 1}Hans Berger Department of Neurology, Jena University Hospital This video article illustrates a comprehensive protocol to detect and quantify all stages of adult hippocampal neurogenesis within the same tissue section. We elaborated a method to overcome the limitations of indirect multiple immunofluorescence that arise when suitable antibodies from different host species are unavailable.

• Bioengineering

  
  Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging Felista L. Tansi*¹, Ronny Rüger*², Markus Rabenhold², Frank Steiniger³, Alfred Fahr², Ingrid Hilger^{1 1}Experimental Radiology, Institute of Diagnostic and Interventional Radiology I, Jena University Hospital, ²Department of Pharmaceutical Technology, Friedrich-Schiller-University Jena, ³Center for Electron Microscopy, Jena University Hospital The use of fluorophores for in vivo imaging can be greatly limited by opsonization, rapid clearance, low detection sensitivity and cytotoxic effects on the host. Encapsulation of fluorophores in liposomes by film hydration and extrusion leads to fluorescence quenching and protection which enables in vivo imaging with high detection sensitivity.

• Medicine

  
  Monitoring of Systemic and Hepatic Hemodynamic Parameters in Mice Chichi Xie^1,3, Weiwei Wei¹, Tao Zhang¹, Olaf Dirsch², Uta Dahmen^{1 1}Department of General, Visceral and Vascular Surgery, Jena University Hospital, ²Institute of Pathology, Jena University Hospital, ³Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University This film demonstrates how to acquire systemic and hepatic hemodynamics in mice. The whole monitoring includes acquisition of vital parameters, systemic blood pressure, central venous pressure, common hepatic artery flow rate, and portal vein pressure as well as the portal flow rate in mice.

• Neuroscience

  
  In vivo Imaging of Optic Nerve Fiber Integrity by Contrast-Enhanced MRI in Mice Stefanie Fischer*¹, Christian Engelmann*², Karl-Heinz Herrmann³, Jürgen R. Reichenbach³, Otto W. Witte¹, Falk Weih², Alexandra Kretz¹, Ronny Haenold^{2 1}Hans Berger Department of Neurology, Jena University Hospital, ²Immunology, Leibniz Institute for Age Research, Fritz Lipmann Institute, Jena, ³Institute of Diagnostic and Interventional Radiology, Medical Physics Group, Jena University Hospital This video illustrates a method, using a clinical 3 T scanner, for contrast-enhanced MR imaging of the naïve mouse visual projection and for repetitive and longitudinal in vivo studies of optic nerve degeneration associated with acute optic nerve crush injury and chronic optic nerve degeneration in knock-out mice (p50^KO).

• Neuroscience

  
  Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo Benedikt Grünewald^1,2, Christian Geis^{1,2 1}Hans Berger Department of Neurology, Jena University Hospital, Jena, Germany, ²Integrated Research and Treatment Center, Center for Sepsis Control and Care (CSCC), Jena University Hospital, Jena, Germany GABAergic presynaptic inhibition is a powerful inhibitory mechanism in the spinal cord important for motor and sensory signal integration in spinal cord networks. Underlying primary afferent depolarization can be measured by recording of dorsal root potentials (DRP). Here we demonstrate a method of in vivo recording of DRP in mice.