2 articles published in JoVE
Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues Julia Barthelmes*1, Nadja Tafferner*2, Jennifer Kurz2, Natasja de Bruin2, Michael J. Parnham2, Gerd Geisslinger1, Susanne Schiffmann2 1Institute of Clinical Pharmacology, Goethe University Hospital Frankfurt, 2Project Group for Translational Medicine & Pharmacology, Fraunhofer IME This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.
Shock Wave Application to Cell Cultures Johannes Holfeld1, Can Tepeköylü1, Radoslaw Kozaryn1, Wolfgang Mathes1, Michael Grimm1, Patrick Paulus2 1Department of Cardiac Surgery, Innsbruck Medical University, 2Clinic of Anesthesiology, Intensive Care Medicine and Pain Therapy, Goethe-University Hospital Shock waves nowadays are well known for their regenerative effects. Therefore in vitro experiments are of increasing interest. We therefore developed a model for in vitro shock wave trials (IVSWT) that enables us to mimic in vivo conditions thereby avoiding distracting physical effects.