University Medical Center Giessen and Marburg 1 article published in JoVE Immunology and Infection Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages Anna Lena Jung1, Kerstin Hoffmann1, Christina E. Herkt1, Christine Schulz1, Wilhelm Bertrams1, Bernd Schmeck1,2,3 1Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg Here, we describe the purification of Legionella pneumophila (L. pneumophila) outer membrane vesicles (OMVs) from liquid cultures. These purified vesicles are then used for the treatment of macrophages to analyze their pro-inflammatory potential.
