3 articles published in JoVE
Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins Isabell-Christin Fiedler1, Arne Weiberg2, Karina van der Linde1 1Department of Biology, Regensburg University, 2Department of Biology, Ludwig-Maximilians University of Munich This work describes the cloning of an Ustilago maydis Trojan horse strain for the in situ delivery of secreted maize proteins into three different types of maize tissues.
The Mouse Isolated Perfused Kidney Technique Jan Czogalla1, Frank Schweda2, Johannes Loffing1 1Institute of Anatomy, Swiss National Centre of Competence in Research Kidney, University of Zürich, 2Department of Physiology, University of Regensburg The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells Christian Schütz1, Martin Fleck2,3, Jonathan P. Schneck1, Mathias Oelke1 1Department of Pathology, Institute of Cell Engineering, Johns Hopkins University, 2Department of Internal Medicine I, University of Regensburg, 3Department of Rheumatology, Asklepios Medical Center Guidelines are presented for the generation of killer artificial antigen presenting cells, KaAPC, an efficient tool for in vitro depletion of human antigen-specific T cells and an alternative solution to cellular immunotherapy for the treatment of T cell-mediated autoimmune diseases in an antigen-specific fashion without compromising the remaining immune system.