2 articles published in JoVE
Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain Claudia Pösel*1, Karoline Möller*1, Johannes Boltze1,2, Daniel-Christoph Wagner1, Gesa Weise1,3 1Ischemia Research Unit, Fraunhofer Institute for Cell Therapy and Immunology, 2Fraunhofer Research Institution for Marine Biotechnology, University of Lubeck, 3Department of Neurology, University of Leipzig Inflammation plays a central role in the pathogenesis of ischemic stroke. Increasing evidence suggests that it acts as a double-edged sword which exacerbates early brain injury, but also contributes to later repair. This protocol describes the isolation of immune cells from the ischemic brain and their subsequent flow cytometric phenotyping.
A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo Thilo L. Schenck1, Myra N. Chávez1, Alexandru P. Condurache2, Ursula Hopfner1, Farid Rezaeian3, Hans-Günther Machens1, José T. Egaña1,4 1Department of Plastic Surgery and Hand Surgery, University Hospital rechts der Isar, Technische Universität München, 2Institute for Signal Processing, University of Lübeck, 3Department of Plastic Surgery and Hand Surgery, University Hospital Zürich, 4FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.