Albert Ludwigs University Freiburg 4 articles published in JoVE Medicine A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease. Babak Saravi*1,2, Gernot Lang*2, Sibylle Grad1, Mauro Alini1, R. Geoff Richards1,2, Hagen Schmal2, Norbert Südkamp2, Zhen Li1 1AO Research Institute Davos, Davos, Switzerland, 2Department of Orthopedics and Trauma Surgery, Medical Centre-Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg, Germany This protocol presents a novel experimental model of proinflammatory, degenerative bovine organ culture to simulate early-stage intervertebral disc degeneration. Chemistry Covalent Attachment of Single Molecules for AFM-based Force Spectroscopy Adrianna Kolberg1, Christiane Wenzel1, Thorsten Hugel1,3, Markus Gallei2, Bizan N. Balzer1,3 1Institute of Physical Chemistry, Albert-Ludwigs-Universität Freiburg, 2Chair in Polymer Chemistry, Saarland University, 3Cluster of Excellence livMatS at FIT - Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg Covalent attachment of probe molecules to atomic force microscopy (AFM) cantilever tips is an essential technique for the investigation of their physical properties. This allows us to determine the stretching force, desorption force and length of polymers via AFM-based single molecule force spectroscopy with high reproducibility. Biochemistry High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis Catrin Swantje Müller1, Wolfgang Bildl1, Norbert Klugbauer2, Alexander Haupt1, Bernd Fakler1,3, Uwe Schulte1,3,4 1Institute of Physiology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany A versatile cryoslicing BN-MS protocol using a microtome is presented for high-resolution complexome profiling. Behavior Non-Invasive Electrical Brain Stimulation Montages for Modulation of Human Motor Function Marco Curado1, Brita Fritsch1, Janine Reis1 1Department of Neurology, Albert Ludwigs University Freiburg Non-invasive electrical brain stimulation can modulate cortical function and behavior, both for research and clinical purposes. This protocol describes different brain stimulation approaches for modulation of the human motor system.
