University Medicine Rostock 1 article published in JoVE Cancer Research Creation and Maintenance of a Living Biobank - How We Do It Florian Bürtin1, Stephanie Matschos2, Friedrich Prall3, Christina S. Mullins2, Mathias Krohn2, Michael Linnebacher2 1Department of General, Visceral, Vascular and Transplantation Surgery, University Medical Center Rostock, University of Rostock, 2Molecular Oncology and Immunotherapy, Department of General, Visceral, Vascular and Transplantation Surgery, University Medical Center Rostock, 3Institute of Pathology, University Medical Center Rostock In the following work, we describe the consecutive steps necessary for the establishment of a large biobank of colorectal and pancreatic cancer.