Deutsches Zentrum fur Neurodegenerative Erkrankungen (DZNE) 1 article published in JoVE Neuroscience In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures Eissa Alfadil1, Frank Bradke1, Sebastian Dupraz1 1Laboratory of Axon Growth and Regeneration, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.
