University Heart and Vascular Center Hamburg (UHZ) 2 articles published in JoVE Medicine Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels Yvonne Schneeberger1, Andreas Schaefer1, Hermann Reichenspurner1, Lenard Conradi1 1Department of Cardiovascular Surgery, University Heart and Vascular Center Hamburg This protocol demonstrates in detail how to perform fully endoscopic mitral valve surgery (EMS) with percutaneous cannulation of the groin vessels, using a percutaneous plug-based vascular closure device. Fundamental steps and useful instructions are described in detail for each step. Medicine Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion Christoph R. Behem1, Till Friedheim1, Sabine H. Wipper2, Hans O. Pinnschmidt3, Michael F. Graessler1, Catharina Gaeth4, Hannes Holthusen1, Adina Rapp5, Timo Suntrop1, Josephina Haunschild6, Christian D. Etz6, Constantin J. C. Trepte1 1Department of Anesthesiology, Center of Anesthesiology and Intensive Care Medicine, University Medical Center Hamburg-Eppendorf, 2University Department for Vascular Surgery and Department of Operative Medicine, Medical University of Innsbruck, 3Department of Medical Biometry and Epidemiology, University Medical Center Hamburg-Eppendorf, 4Department of Vascular Medicine, University Heart and Vascular Center Hamburg (UHZ), 5Department of Cardiology, Rostock University Medical Center, 6University Department for Cardiac Surgery, Heart Center Leipzig Spinal cord microcirculation plays a pivotal role in spinal cord injury. Most methods do not allow real-time assessment of spinal cord microcirculation, which is essential for the development of microcirculation-targeted therapies. Here, we propose a protocol using Laser-Doppler-Flow Needle probes in a large animal model of ischemia/reperfusion.