3 articles published in JoVE
In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells Joan Repullés1,2, Mariona Terradas1,3,4, Gemma Fuster5,6,7, Anna Genescà1, Teresa Anglada1 1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic This protocol provides experimental in vitro tools to evaluate the transformation of human mammary cells. Detailed steps to follow-up cell proliferation rate, anchorage-independent growth capacity, and distribution of cell lineages in 3D cultures with basement membrane matrix are described.
Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals Sara Olivera-Ardid*1, Nailya Khasbiullina*2,3, Alexey Nokel3, Andrey Formanovsky2, Inna Popova2, Tatiana Tyrtysh2, Roman Kunetskiy2, Nadezhda Shilova2, Nicolai Bovin2,4, Daniel Bello-Gil1, Rafael Mañez1,5 1Infectious Pathology and Transplantation Division, Bellvitge Biomedical Research Institute (IDIBELL), 2Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry (IBCh), Russian Academy of Sciences, 3Semiotik LLC, 4Auckland University of Technology, 5Intensive Care Department, Bellvitge University Hospital This work shows the potential of printed glycan array (PGA) technology for the analysis of circulating anti-carbohydrate antibodies in small animals.
Basic Caenorhabditis elegans Methods: Synchronization and Observation Montserrat Porta-de-la-Riva1,2, Laura Fontrodona1, Alberto Villanueva1,2, Julián Cerón1,2 1Department of Cancer and Human Molecular Genetics, Bellvitge Institute for Biomedical Research, 2C. elegans Core Facility, Bellvitge Institute for Biomedical Research The easiness of maintaining and propagating the nematode C. elegans make it a nice model organism to work with. The possibility of synchronizing worms allows the work with a significant amount of subjects at the same developmental stage, what facilitates the study of one particular process in many animals.