University of Valladolid 2 articles published in JoVE Environment Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples José Gonçalves1,2,3, Priscilla Gomes da Silva4,5,6, Tom Koritnik3, Martin Bosilj3, Andrés Torres-Franco1,2, Israel Diaz1,2, Elisa Rodriguéz1,2, Eva Marcos2,3, João R. Mesquita4,5, Pedro García-Encina1,2 1Institute of Sustainable Processes, University of Valladolid, 2Department of Chemical Engineering and Environmental Technology, University of Valladolid, 3Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, 4ICBAS - School of Medicine and Biomedical Sciences, Porto University, 5Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR) This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization. Engineering Effective Analysis of Human Exposure Conditions with Body-worn Dosimeters in the 2.4 GHz Band Silvia de Miguel-Bilbao1, Juan Blas2, Victoria Ramos1 1Telemedicine and e-Health Research Unit, Instituto de Salud Carlos III, 2Signal Theory and Communications, and Telematic Engineering Department, Universidad de Valladolid This study describes a protocol to measure exposure levels in the 2.4 GHz band, avoiding the uncertainties caused by the use of personal exposimeters as measuring devices. These alterations of the exposure levels should be taken into account, especially in compliance testing, where exposure limits are defined from non-perturbed data.
