University of Zaragoza

2 articles published in JoVE

• Biochemistry

  
  Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.) Inés Medina-Lozano*^1,4, Juan Ramón Bertolín*^2,4, Raquel Zufiaurre³, Aurora Díaz^{1,4 1}Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), ²Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), ³Dpto Química Analítica, Escuela Politécnica Superior (Universidad de Zaragoza), ⁴Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza) We present a fast and reliable method to quantify vitamin C in Lactuca spp. using UPLC-UV, potentially transferable to other plants. The key steps are the sample preparation and vitamin C extraction under stable conditions, the reduction of dehydroascorbic acid to ascorbic acid and the optimization of the chromatographic procedure.

• Immunology and Infection

  
  Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice Santiago Uranga¹, Dessislava Marinova¹, Carlos Martin¹, Nacho Aguilo^{1 1}Department of Microbiology, University of Zaragoza We herein detail the methodology followed to compare protective efficacy and lung immune response induced by intranasal and subcutaneous immunization with BCG in mouse model. Our results show the benefits of pulmonary vaccination and suggest a role for IL17-mediated response in vaccine-induced protection.