2 articles published in JoVE
A Freeze-Thawing Method to Prepare Chitosan-Poly(vinyl alcohol) Hydrogels Without Crosslinking Agents and Diflunisal Release Studies María Dolores Figueroa-Pizano1, Itziar Vélaz2, María Elisa Martínez-Barbosa1 1Department of Polymers and Materials Research, University of Sonora, 2Department of Chemistry, Faculty of Sciences, University of Navarra The freezing-thawing method is used to produce chitosan-poly(vinyl alcohol) hydrogels without crosslinking agents. For this method, it is important to consider the freezing conditions (temperature, number of cycles) and polymer ratio, which can affect the properties and applications of the obtained hydrogels.
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells Alain Casanova*1, Shyan H. Low*1, Mario Emmenlauer*1,4, Raquel Conde-Alvarez*1,3, Suzana P. Salcedo2, Jean-Pierre Gorvel2, Christoph Dehio1 1Focal Area Infection Biology, Biozentrum, University of Basel, 2Centre d’Immunologie de Marseille-Luminy, Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, 3Departmento de Microbiologìa and Instituto de Salud Tropical, Universidad de Navarra, 4BioDataAnalysis GmbH Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.